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Search Brenda Oppert - All Publications 78 found
Article Link Klocko, A.L., R. Meilan, R.R. James, V. Viswanath, C. Ma, P. Payne, L. Miller, J.S. Skinner, B. Oppert, G.A. Cardineau, and S.H. Strauss. 2014. Bt-Cry3Aa transgene expression reduces insect damage and improves growth in field-grown hybrid poplar. Can. J. For. Res. 44: 28-35.
Article Link Semashko, T.A., E.A. Vorotnikova, V.F. Sharikova, K.S. Vinokurov, Y.A. Smirnova, Y.E. Dunaevsky, M.A. Belozersky, B. Oppert, E.N. Elpidina. 2014. Selective chromogenic and fluorogenic peptide substrates for the assay of cysteine peptidases in complex mixtures. Anal. Biochem. 449: 179-187.
 pdf icon PDF  Goptar, I.A., D.A. Shagin, I.A. Shagina, E.S. Mudrik, Y.A. Smirnova, D.P. Zhuzhikov, Y.E. Dunaevsky, B.S. Oppert, I.Y. Filippova, and E.N. Elipidina. 2013. A digestive prolyl carboxypeptidase in Tenebrio molitor larvae. Insect Biochem. Mol. Biol. 43: 501:509.
 pdf icon PDF  Oppert, B., and T.D. Morgan. 2013. Improved high-throughput bioassay for Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). J. Stored Prod. Res. 52: 68-73.
 pdf icon PDF  Gao, Y., B.S. Oppert, J.C. Lord, C. Liu, and Z. Lei. 2012. Bacillus thuringiensis Cry3Aa toxin increases the susceptibility of Crioceris quatuordecimpunctata to Beauveria bassiana infection. J. Invertebr. Pathol. 109: 260-263.
 pdf icon PDF  Goptar, I.A., T.A. Semashko, S.A. Danilenko, E.N. Lysogorskaya, E.S. Oksenoit, D.P. Zhuzhikov, M.A. Belozersky, Y.E. Dunaevsky, B. Oppert, I.Y. Filippova, and E.N. Elpidina. 2012. Cysteine digestive peptidases function as post-glutamine cleaving enzymes in tenebrionid stored-product pests. Comp. Biochem. Physiol. Part B 161: 148-154.
 pdf icon PDF  Oppert, B., A.G. Martynov, and E.N. Elpidina. 2012. Bacillus thuringiensis Cry3Aa protoxin intoxication of Tenebrio molitor induces widespread changes in the expression of serine peptidase transcripts. Comp. Biochem. Physiol. Part D 7: 233-243.
 pdf icon PDF  Oppert, B.S., S.E. Dowd, P. Bouffard, L. Li, A. Conesa, M.D. Lorenzen, M. Toutges, J. Marshall, D.L. Huestis, J.A. Fabrick, C. Oppert, and J.L. Jurat-Fuentes. 2012. Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin. PLoS ONE 7(e34624), 12 pp.
 pdf icon PDF  Valadez-Lira, J.A., J.M. Alcocer-Gonzalez, G. Damas, G. Nuñez-Mejiá, B. Oppert, C. Rodriguez-Padilla, and P. Tamez-Guerra. 2012. Comparative evaluation of phenoloxidase activity in different larval stages of four lepidopteran pests after exposure to Bacillus thuringiensis. J. Insect Sci. 12(80),11 pp.
Article Link Yao, J., L.L. Buschman, B. Oppert, C. Khajuria, and K.Y. Zhu. 2012. Characterization of cDNAs encoding serine proteases and their transcriptional responses to Cry1Ab protoxin in the gut of Ostrinia nubilalis larvae. PloS ONE 7(8): e44090.
 pdf icon PDF  Gao, Y., J. L. Jurat-Fuentes, B. Oppert, J.A. Fabrick, C. Liu, J. Gao, and Z. Lei. 2011. Increased toxicity of Bacillus thuringiensis Cry3Aa against Crioceris quatuordecimpunctata, Phaedon brassicae and Colaphellus bowringi by a Tenebrio molitor cadherin fragment. Pest Manag. Sci. 67: 1076-1081.
 pdf icon PDF  Oppert, B., T.D. Morgan, and K.J. Kramer. 2011. Efficacy of Bacillus thuringiensis Cry3Aa protoxin and protease inhibitors against coleopteran storage pests. Pest Manag. Sci. 67: 568-573.
Article Link Toutges, M.J., K.L. Hartzer, J.C. Lord, and B.S. Oppert. 2011. Evaluation of reference genes for quantitative polymerase chain reaction across life cycle stages and tissue types of Tribolium castaneum. J. Agric. Food Chem. 58: 8948-8951.
 pdf icon PDF  Willis, J.D., B.S. Oppert, C. Oppert, W.E. Klingeman, and J.L. Jurat-Fuentes. 2011. Identification, cloning, and expression of a GHF9 cellulase from Tribolium castaneum (Coleoptera: Tenebrionidae). J. Insect Physiol. 57: 300-306.
 pdf icon PDF  Dubovenko, A.G., Y.E. Dunaevsky, M.A. Belozersky, B. Oppert, J.C. Lord, and E.N. Elpidina. 2010. Trypsin-like proteins of the fungi as possible markers of pathogenicity. Fungal Biol. 114: 151-159.
 pdf icon PDF  Gao, Y., Y. Hu, Q. Fu, J. Zhang, B.S. Oppert, F. Lai, and Z. Zhang. 2010. Screen of Bacillus thuringiensis toxins for transgenic rice to control Sesamia inferens and Chilo suppressalis. J. Invertebr. Pathol. 105: 11-15.
 pdf icon PDF  Liu, C., Y. Gao, C. Ning, K. Wu, B.S. Oppert, and Y. Gao. 2010. Antisera-mediated in vivo reduction of Cry1Ac toxicity in Helicoverpa armigera. J. Insect Physiol. 56: 718-724.
 pdf icon PDF  Lord, J.C., K. Hartzer, M. Toutges, and B. Oppert. 2010. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge. J. Microbiol. Meth. 80: 219-221.
 pdf icon PDF  Oppert B.S., E.N. Elpidina, M. Toutges, and S. Mazumdar-Leighton. 2010. Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors. Comp. Biochem. Physiol. Part D 5: 280-287.
 pdf icon PDF  Oppert, B.S. 2010. Rapid bioassay to screen potential biopesticides in Tenebrio molitor larvae. Biopestic. Int. 6: 67-73.
 pdf icon PDF  Oppert, B.S., R.T. Ellis, and J. Babcock. 2010. Effects of Cry1F and Cry34Ab1/35Ab1 on storage pests. J. Stored Prod. Res. 46: 143-148.
 pdf icon PDF  Oppert, C., W.E. Klingeman, J.D. Willis, B. Oppert, and J.L. Jurat-Fuentes. 2010. Prospecting for cellulolytic activity in insect digestive fluids. Comp. Biochem. Physiol. Part B 155: 145-154.
 pdf icon PDF  Willis, J.D., W.E. Klingeman, C. Oppert, B.S. Oppert, and J.L. Jurat-Fuentes. 2010. Characterization of cellulolytic activity from digestive fluids of Dissosteira carolina (Orthoptera: Acrididae). Comp. Biochem. Physiol. Part B 157: 267-272.
Article Link Crook, D.J., S. Prabhakar, and B. Oppert. 2009. Protein digestion in larvae of the red oak borer Enaphalodes rufulus. Physiol. Entomol. 34: 152-157.
 pdf icon PDF  Fabrick, J., C. Oppert, M.D. Lorenzen, K. Morris, B. Oppert, and J.L. Jurat-Fuentes. 2009. A novel Tenebrio molitor cadherin is a functional receptor for Bacillus thuringiensis Cry3Aa toxin. J. Biol. Chem. 284: 18401-18410.
 pdf icon PDF  Huestis, D.L., B. Oppert, and J.L. Marshall. 2009. Geographic distributions of Idh-1 alleles in a cricket are linked to differential enzyme kinetic performance across thermal environments. BMC Evol. Biol. 9(113), 12 pp.
 pdf icon PDF  Liu, C., K. Wu, Y. Wu, Y. Gao, C. Ning, and B. Oppert. 2009. Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. J. Insect Physiol. 55: 686-693.
 pdf icon PDF  Marshall, J.L., D.L. Huestis, Y. Hiromasa, S. Wheeler, C. Oppert, S.A. Marshall, J.M. Tomich, and B. Oppert. 2009. Identification, RNAi knockdown, and functional analysis of an ejaculate protein that mediates a postmating, prezygotic phenotype in a cricket. PloS ONE 4(e7537), 10 pp.
 pdf icon PDF  Morris, K., M.D. Lorenzen, Y. Hiromasa, J.M. Tomich, C. Oppert, E.N. Elpidina, K.Vinokurov, J.L. Jurat-Fuentes, J. Fabrick, and B. Oppert. 2009. Tribolium castaneum larval gut transcriptome and proteome: a resource for the study of the coleopteran gut. J. Proteome Res. 8: 3889-3898.
 pdf icon PDF  Vinokurov, K.S., E.N. Elpidina, D.P. Zhuzhikov, B. Oppert, D. Kodrik, and F. Sehnal. 2009. Digestive proteolysis organization in two closely related Tenebrionid beetles: Red flour beetle (Tribolium castaneum)and confused flour beetle (Tribolium confusum). Arch. Insect Biochem. Physiol. 70: 254-279.
 pdf icon PDF  Goiptar, I.A., I.Y. Filippova, E.N. Lysogorskaya, E.S. Oksenoit, K.S. Vinokurov, D.P. Zhuzhikov, N.V. Bulushova, I.A. Zalunin, Y.E. Dunaevsky, M.A. Belozersky, B. Oppert, and E.N. Elpidina. 2008. Localization of post-proline cleaving peptidases in Tenebrio molitor larval midgut. Biochimie 90: 508-514.
Article Link Janarthanan, S., P. Suresh, G. Radke, T.D. Morgan, and B. Oppert. 2008. Arcelins from an Indian wild pulse, Lablab purpureus, and insecticidal activity in storage pests. J. Agric. Food Chem. 56: 1676-1682.
 pdf icon PDF  Park, Y., J. Aikins, L.J. Wang, R.W. Beeman, B. Oppert, J.C. Lord, S.J. Brown, M.D. Lorenzen, S. Richards, G.M. Weinstock, and R.A. Gibbs. 2008. Analysis of transcriptome data in the red flour beetle, Tribolium castaneum. Insect Biochem. Mol. Biol. 38: 380-386.
 pdf icon PDF  Tamez-Guerra, P., J.A. Valadez-Lira, J.M. Alcocer-Gonzalez, B. Oppert, R. Gomez-Flores, R. Tamez-Guerra, and C. Rodriguez-Padilla. 2008. Detection of genes encoding antimicrobial peptides in Mexican strains of Trichoplusia ni (Hübner) exposed to Bacillus thuringiensis. J. Invertebr. Pathol. 98: 218-227.
Article Link Tribolium Sequencing Consortium (includes R.W. Beeman, M.D. Lorenzen, B. Oppert, J. Lord, K. Kramer, Y. Arakane). 2008. The genome of the model beetle and pest Tribolium castaneum. Nature 452: 949-955.
 pdf icon PDF  Karumbaiah, L., B. Oppert, J.L. Jurat-Fuentes, and M.J. Adang. 2007. Analysis of midgut proteinases from Bacillus thuringiensis-susceptible and -resistant Heliothis virescens (Lepidoptera: Noctuidae). Comp. Biochem. Physiol. Part B 146: 139-146.
 pdf icon PDF  Li, H., L.L. Buschman, F. Huang, K.Y. Zhu, B. Bonning, and B. Oppert. 2007. DiPel-selected Ostrinia nubilalis larvae are not resistant to transgenic corn expressing Bacillus thuringiensis Cry1Ab. J. Econ. Entomol. 100: 1862-1870.
            Li, H., L.L. Buschman, K.Y. Zhu, F. Huang, and B. Oppert. 2007. Resistance to Bacillus thuringiensis endotoxins in the European corn borer, Ostrinia nubilalis. Biopestic. Int. 3: 96-107.
 pdf icon PDF  Prabhakar, S., M.S. Chen, E.N. Elpidina, K.S. Vinokurov, C.M. Smith, J. Marshall, and B. Oppert. 2007. Sequence analysis and molecular characterization of larval midgut cDNA transcripts encoding peptidases from the yellow mealworm, Tenebrio molitor L. Insect Mol. Biol. 16: 455-468.
 pdf icon PDF  Oppert, B. 2006. Two-dimensional analysis of proteinase activity. J. Biochem. Biophys. Methods 67: 173-179.
 pdf icon PDF  Oppert, B., P. Walters, and M. Zuercher. 2006. Digestive proteinases of the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae). Bull. Entomol. Res. 96: 167-172.
 pdf icon PDF  Tamez-Guerra, P.G. Damas, M.M. Iracheta, B. Oppert, R. Gomez-Flores, and C. Rodriguez-Padilla. 2006. Differences in susceptibility and physiological fitness of Mexican field Trichoplusia ni strains exposed to Bacillus thuringiensis. J. Econ. Entomol. 99: 937-945.
 pdf icon PDF  Vinokurov, K.S., E.N. Elpidina, B. Oppert, S. Prabhakar, D.P. Zhuzhikov, Y.E. Dunaevsky, and M.A. Belozersky. 2006. Fractionation of digestive proteinases from Tenebrio molitor (Coleoptera: Tenebrionidae) larvae and role in protein digestion. Comp. Biochem. Physiol. Part B 145: 138-146.
 pdf icon PDF  Vinokuroz, K.S., E.N. Elpidina, B. Oppert, S. Prabhakar, D.P. Zhuzhikov, Y.E. Dunaevsky, and M.A. Belozersky. 2006. Diversity of digestive proteinases in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. Comp. Biochem. Physiol. Part B 145: 126-137.
 pdf icon PDF  Elpidina, E.N., T.A. Tsybina, Y.E. Dunaevsky, M.A. Belozersky, D.P. Zhuzhikov, and B. Oppert. 2005. A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae. Biochimie 87: 771-779.
 pdf icon PDF  Li, H., B. Oppert, R.A. Higgins, F. Huang, L.L. Buschman, and K.Y. Zhu. 2005. Susceptibility of Dipel-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae) to individual Bacillus thuringiensis protoxins. J. Econ. Entomol. 98: 1333-1340.
 pdf icon PDF  Li, H., B. Oppert, R.A. Higgins, F. Huang, L.L. Buschman, J. Gao, and K.Y. Zhu. 2005. Characterization of cDNAs encoding three trypsin-like proteinases and mRNA quantitative analysis in Bt-resistant and -susceptible strains of Ostrinia nubilalis. Insect Biochem. Mol. Biol. 35: 847-860.
 pdf icon PDF  Oppert, B., T.D. Morgan, K. Hartzer, and K.J. Kramer. 2005. Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Comp. Biochem. Physiol. Part C 140: 53-58.
 pdf icon PDF  Tsybina, T.A., Y.E. Dunaevsky, M.A. Belozersky, D.P. Zhuzhikov, B. Oppert, and E.N. Elpidina. 2005. Digestive proteinases of yellow mealworm (Tenebrio molitor) larvae: purification and characterization of a trypsin-like proteinase. Biochemistry (Moscow) 70: 300-305.
 pdf icon PDF  Vinokurov, K.S., B. Oppert, and E.N. Elpidina. 2005. An overlay technique for postelectrophoretic analysis of proteinase spectra in complex mixtures using p-nitroanilide substrates. Anal. Biochem. 337: 164-166.
 pdf icon PDF  Zhu, Y.C., X. Liu, A.S. Maddur, B. Oppert, and M.S Chen. 2005. Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [Mayetiola destructor (say)]. Insect Biochem. Mol. Biol. 35: 23-32.
 pdf icon PDF  Li, H., B. Oppert, R.A. Higgins, F. Huang, K.Y. Zhu, and L.L. Buschman. 2004. Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae). Insect Biochem. Mol. Biol. 34: 753-762.
 pdf icon PDF  Li, H., J. González-Cabrera, B. Oppert, J. Ferré, R.A. Higgins, L.L. Buschman, G.A. Radke, K.Y. Zhu, and F. Huang. 2004. Binding analyses of Cry1Ab and Cry1Ac with membrane vesicles from Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis. Biochem. Biophys. Res. Comm. 323: 52-57.
 pdf icon PDF  Candas, M., O. Loseva, B. Oppert, P. Kosaraju, and L.A. Bulla, Jr. 2003. Insect resistance to Bacillus thuringiensis: Alterations in the Indianmeal moth larval gut proteome. Mol. Cell. Proteomics 2: 19-28.
 pdf icon PDF  Li, H., B. Oppert, K.Y. Zhu, R.A. Higgins, F. Huang, and L.L. Buschman. 2003. Transgenic plants expressing Bacillus thuringiensis delta-endotoxins. Entomologia Sinica 10: 155-166.
 pdf icon PDF  Oppert, B., T.D. Morgan, K. Hartzer, B. Lenarcic, K. Galesa, J. Brzin, V. Turk, K. Yoza, K. Ohtsubo, and K.J. Kramer. 2003. Effects of proteinase inhibitors on digestive proteinases and growth of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Comp. Biochem. Physiol. Part C 134: 481-490.
 pdf icon PDF  Zhu, Y.C., F. Zeng, and B. Oppert. 2003. Molecular cloning of trypsin-like cDNAs and comparison of proteinase activities in the salivary glands and gut of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae). Insect Biochem. Mol. Biol. 33: 889-899.
 pdf icon PDF  Oppert, B., K. Hartzer, and M. Zuercher. 2002. Digestive proteinases in Lasioderma serricorne (Coleoptera: Anobiidae). Bull. Entomol. Res. 92: 331-336.
 pdf icon PDF  Herrero, S., B. Oppert, and J. Ferre. 2001. Different mechanisms of resistance to Bacillus thuringiensis toxins in the Indianmeal moth. Appl. Environ. Microbiol. 67: 1085-1089.
 pdf icon PDF  Oppert, B., K. Hartzer, and C.M. Smith. 2000. Digestive proteinases of alfalfa weevil, Hypera postica, (Gyllenhal) (Coleoptera: Curculionidae). Trans. Kansas Acad. Sci. 103: 99-110.
 pdf icon PDF  Oppert, B., R. Hammel, J.E. Throne, and K.J. Kramer. 2000. Fitness costs of resistance to Bacillus thuringiensis in the Indianmeal moth, Plodia interpunctella. Entomol. Exp. Appl. 96: 281-287.
            Oppert, B.S. 2000. Transgenic plants expressing enzyme inhibitors and the prospects for biopesticide development, pp. 83-95. In: O. Koul and G.S. Dhaliwal (Eds.), Phytochemical Biopesticides. Harwood Academic Publishers, Amsterdam, The Netherlands.
 pdf icon PDF  Zhu, Y.C., B. Oppert, K.J. Kramer,W.H. McGaughey, and A.K. Dowdy. 2000. cDNA sequence, mRNA expression and genomic DNA of trypsinogen from the Indianmeal moth, Plodia interpunctella. Insect Mol. Biol. 9: 19-26.
 pdf icon PDF  Zhu, Y.C., K.J. Kramer, B. Oppert, and A.K. Dowdy, 2000. cDNAs of aminopeptidase-like protein genes from Plodia interpunctella strains with different susceptibilities to Bacillus thuringiensis toxins. Insect Biochem. Mol. Biol. 30: 215-224.
 pdf icon PDF  Huang, F., K.Y. Zhu, L.L. Buschman, R.A. Higgins, and B. Oppert. 1999. Comparison of midgut proteinases in Bacillus thuringiensis-susceptible and -resistant European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). Pestic. Biochem. Physiol. 65: 132-139.
 pdf icon PDF  Oppert, B. 1999. Protease interactions with Bacillus thuringiensis insecticidal toxins. Arch. Insect Biochem. Physiol. 42: 1-12.
            Reeck, G., B. Oppert, M. Denton, M. Kanost, J.E. Baker, and K.J. Kramer. 1999. Insect proteinases, pp. 125-148. In: V. Turk (Ed.), Proteases: new perspectives. Birkhauser Verlag, Basel, Switzerland.
 pdf icon PDF  Johnson, D.E., B. Oppert, and W.H. McGaughey. 1998. Spore coat protein synergizes Bacillus thuringiensis crystal toxicity for the Indianmeal moth (Plodia interpunctella). Curr. Microbiol. 36: 278-282.
            McGaughey, W.H., and B. Oppert. 1998. Mechanisms of insect resistance to Bacillus thuringiensis toxins. Isr. J. Entomol. XXXII: 1-14.
            Oppert, B.S., and K.J. Kramer. 1998. Economical use of substrates in enzyme activity blots. American Biotech. Lab, pp. 16-20.
            Oppert, B., K.J. Kramer, and W.H. McGaughey. 1997. Rapid microplate assay for substrates and inhibitors of proteinase mixtures. BioTechniques 23: 70-72.
            Oppert, B., K.J. Kramer, and W.H. McGaughey. 1997. Insect resistance to Bacillus thuringiensis toxins. The Chemist 74: 7-10.
 pdf icon PDF  Oppert, B., K.J. Kramer, R.W. Beeman, D.E. Johnson, and W.H. McGaughey. 1997. Proteinase-mediated insect resistance to Bacillus thuringiensis toxins. J. Biol. Chem. 272: 23473-23476.
 pdf icon PDF  Zhu, Y., B. Oppert, K.J. Kramer, W.H. McGaughey, and A.K. Dowdy. 1997. cDNAs for a chymotrypsinogen-like protein from two strains of Plodia interpunctella. Insect Biochem. Mol. Biol. 27: 1027-1037.
 pdf icon PDF  Oppert, B., K.J. Kramer, D.E. Johnson, S.J. Upton, and W.H. McGaughey. 1996. Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin. Insect Biochem. Mol. Biol. 26: 571-583.
            Oppert, B., K.J. Kramer, D.E. Johnson, S.C. MacIntosh, and W.H. McGaughey. 1994. Altered protoxin activation by midgut enzymes from a Bacillus thuringiensis resistant strain of Plodia interpunctella. Biochem. Biophys. Res. Comm. 198: 940-947.
            Morgan, T.D., B. Oppert, T.H. Czapla, and K.J. Kramer. 1993. Avidin and streptavidin as insecticidal and growth inhibiting dietary proteins. Entomol. Exp. Appl. 69: 97-108.
            Oppert, B., T.D. Morgan, C. Culbertson, and K.J. Kramer. 1993. Dietary mixtures of cysteine and serine proteinase inhibitors exhibit synergistic toxicity toward the red flour beetle, Tribolium castaneum. Comp. Biochem. Physiol. Part C 105: 379-385.

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